Insulating jackets thermally protect kidneys in an ex vivo model of second warm ischemia

BackgroundKidney transplantation is the current optimal treatment for suitable patients with end-stage renal disease. The second warm ischemic time (SWIT) is known to negatively impact delayed graft function, and long-term graft survival, and methods are required to ameliorate the impacts of SWIT on transplantation outcomes. Materials and MethodsThis study primarily focused on determining the effect of a novel thermally insulating jacket on the thermal profile of the human kidney and quantifying the reduction in thermal energy experienced using this device (KPJ (TM)). An ex vivo simulated transplantation model was developed to determine the thermal profiles of non-utilized human kidneys with and without KPJ (TM) (n = 5). Control kidney temperature profiles were validated against the temperature profiles of n = 10 kidneys during clinical kidney transplantation. ResultsUsing the ex-vivo water bath model, the thermally insulated human kidney reached the 15 degrees C metabolic threshold temperature at 44.5 +/- 1.9 min (vs control: 17.3 +/- 1.8 min (p = 0.00172)) and remained within the 18 degrees C threshold until 53.3 +/- 1.3 min (vs control: 20.9 +/- 2.0 min (p = 0.002)). The specific heat capacity of KPJ (TM) protected kidney was four-fold compared to the control kidney. The clinical temperature audit, closely correlated with the water bath model, hence validating this ex-vivo human kidney transplant model. ConclusionIntraoperative thermal protection is a simple and viable method of reducing the thermal injury that occurs during the SWIT and increasing the specific heat capacity of the system. Such technology could easily be translated into clinical kidney transplant practice.