Metabolism Difference Is Involved in Mesosulfuron-Methyl Selectivity between Aegilops tauschii and Triticum aestivum

The acetolactate synthase (ALS) inhibitor mesosulfuron-methyl is currently the only selective herbicide to control Aegilops tauschii in wheat fields; however, the mechanism underlying this selectivity remains unclear. Results showed that the tolerance of Triticum aestivum to mesosulfuron-methyl was much higher than that of A. tauschii. Mesosulfuron-methyl inhibited the in vitro ALS activity of A. tauschii and T. aestivum similarly, but the predicted structural interactions of ALS with mesosulfuronmethyl and induced expression of als were different in the two species. Compared with T. aestivum, A. tauschii was found to absorb more mesosulfuron-methyl and metabolize much less mesosulfuron-methyl. The cytochrome P450 monooxygenase (CYP450) inhibitor, malathion, greatly increased the sensitivity of T. aestivum to mesosulfuron-methyl, while its synergistic effect was smaller in A. tauschii. Finally, 19 P450 genes were selected as candidate genes related with metabolism-based mesosulfuron-methyl selectivity. Collectively, different sensitivities to mesosulfuron-methyl in the two species were likely to be attributed to metabolism variances.