Recent Advances in engineering Corynebacterium glutamicum for production of Lysine

Lysine is an essential amino acid utilised in diverse industrial areas, mostly as animal feed and food. It is synthesized by various organisms but the novel Corynebacterium glutamicum (gram positive, non sporulating bacteria) is selected anticipating to the enormous research. This research has paved a way towards enhancing the lysine productivity. Different strains of Corynebacterium glutamicum utilised many technologies for the enhanced production of L-Lysine. This literature study examines the current status of Llysine industrial production with a focus on technology used for development irrespective of the strain and fermentation technologies through different approach of understanding of provisions (NADPH, Media source, PTS, exploit of RAM, Vector systems and promoters) with use of different methodologies. The technologies were divided into metabolic engineering, conventional gene mutations, optimization of fermentation parameters (RSM, ANN, GA), gene overexpression, gene switching and exploit of CRISPR, electric current. This review presents the methodologies which are superior at manufacturing enhanced amounts of lysine and report the techniques which assist in producing the best yield in collation with the earlier. This also focuses on the genes related to lysine export and provides conviction of utilizing lysine as an animal feed.