Structure of Cadmiull}-Substituted Phospholipase A2 from Agkistrodon halys Pallas at 2.8A Resolution

Abstract: The crystal structure of cadmium-substituted basic PLA2 from Agkistrodon halys Pallas (Agkistrodon blomhoffii Brevicaudus, A. h. Pallas) has been determined to 2.8 A with an R factor of 20.1%. Two cadmium-binding sites were found in the enzyme, one is near His48 (S1), the other is near Asp122 (S2). The ligand geometry of the metal ions is described and the inhibition of the catalytic activity by the metal ion is discussed.