Metagenomics analysis of the effects of Agaricus bisporus mycelia on microbial diversity and CAZymes in compost.

growth alters the lignocellulosic composition and structure of compost. However, it is difficult to differentiate the enzyme activities of mycelia from the wider microbial community owing to the complication of completely speareting the mycelia from compost cultures. Macrogenomics analysis was employed in this study to examine the fermentation substrate of before and after mycelial growth, and the molecular mechanism of substrate utilization by mycelia was elucidated from the perspective of microbial communities and CAZymes in the substrate. The results showed that the relative abundance of mycelia increased by 77.57-fold after mycelial colonization, the laccase content was significantly increased and the lignin content was significantly decreased. Analysis of the CAZymes showed that AA10 family was extremely differentiated. Laccase-producing strains associated with AA10 family were mostly bacteria belonging to and , suggesting that these bacteria may play a synergistic role in lignin decomposition along with mycelia. These findings provide preliminary evidence for the molecular mechanism of compost utilization by mycelia and offer a reference for the development and utilization of strains related to lignocellulose degradation.