Axl, Immune Checkpoint Molecules and HIF Inhibitors from the Culture Broth of Lepista luscina .

Two compounds and were isolated from the culture broth of . This is the first time that compound was isolated from a natural source. The structure of compound was identified via 1D and 2D NMR and HRESIMS data. Compounds and along with 8-nitrotryptanthrin () were evaluated for their biological activities using the A549 lung cancer cell line. As a result, and inhibited the expression of Axl and immune checkpoint molecules. In addition, compounds , and were tested for HIF inhibitory activity. Compound demonstrated statistically significant HIF inhibitory effects on NIH3T3 cells and and against ARPE19 cells.