Experimental Apparatus for Evaluation of Calcium Fluctuations in Cardiomyocytes Derived from Human-Induced Pluripotent Stem Cells
XXVII BRAZILIAN CONGRESS ON BIOMEDICAL ENGINEERING, CBEB 2020(2022)
Abstract
In this work, we developed and tested an experimental apparatus to evaluate calcium fluctuations in cardiomyocytes derived from human-induced pluripotent stem cells (hiPSC-CM). The set-up is composed of a signal module for registration and analysis of the signals and a perfusion chamber. This chamber allows the maintenance of the cells, control of perfusion and temperature, and electric stimulation. The signal module consists of a CCD camera attached to a fluorescence microscope with the appropriate software and hardware for eliciting and recording fluorescent signals originating from hiPSC-CM intracellular Ca+2 concentration changes, under electrical stimulation. We employed this system for analysis of calcium fluctuation from hiPSC-CM cultivated on micro textured polyethylene terephthalate surfaces.
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Key words
Calcium fluctuations, hiPSC-CM, Experimental apparatus, Fluorescence, Surface microtopography
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