Isolation of Lactic Acid Bacteria from Yogurt and the Effect on the Intestinal Microflora in Mice

Lactic acid bacteria (LAB) are typical representatives of probiotics owing to their beneficial effects on the host. They have been widely used in the food industry, animal husbandry, health care, and other fields. In this study, 91 strains were isolated and purified from commercial yogurts. And their functional properties, such as bacteriostatic effects, drug resistances, growth performances, adhesion characteristics, surface hydrophobicity, self-aggregation, and copolymerization, were evaluated. The results showed that all indicators of LAB M3 are good, which was identified as Lactobacillus casei by 16S rDNA sequence analysis. Subsequently, the mice were gavaged with Lactobacillus casei M3, and a high-throughput sequencing technology was employed to detect their feces to evaluate the changes in microbial flora in mice. It found that Lactobacillus casei M3 can intervene in the intestines of mice, improve the abundance of beneficial microflora in reducing the incidence of colitis and preventing obesity, and improve the intestinal microflora. It would have a good application prospect in the field of food industry, health care or medicine.