Number and surface molecular markers of regulatory b cells in peripheral blood of patients with vitiligo

Objective: The aetiology of vitiligo is still unclear, and it is difficult to cure. Method: There are many clinical treatments for vitiligo, but their clinical efficacy varies because of individual differences. Therefore, the detection of regulatory B cells in the peripheral blood of patients with vitiligo has far-reaching significance. To detect the number of B cells and regulatory B cells in peripheral blood and peripheral blood mononuclear cells (PBMCs) of patients with vitiligo and the expression of surface molecular markers, and to analyse the effect of regulatory B cells. The subjects of this experiment were 15 patients with non-segmental progressive vitiligo. Fifteen normal people were uses as the control group. The expression of B cells in peripheral blood was detected by flow cytometry. The expression of CD19 + B cells, CD19+IL-10+B cells, CD19+CD5(high)CD1d(high)B cells and CD19+CD24(high)CD38(high)B cells were detected after the PBMCs were cultured and stimulated. Results: The percentage of CD24+CD5(high)CD38(high)B cells in patients with vitiligo was (3.77 +/- 1.11) %, while in the control group it was (1.13 +/- 0.62) %. The difference between the two groups was statistically significant (P<0.05). Conclusion: The detection of regulatory B cells in the peripheral blood of vitiligo patients in this paper has achieved good analysis results.