Study on the Anticancer Effects of Plasma-Activated Saline Perfusion Based on a Microfluidic System

As a means of applying cold atmospheric plasma (CAP), plasma-activated solutions have received extensive attention for their excellent anticancer effects. However, previous studies have focused mainly on the local injection of plasma-activated solutions for cancer treatment while ignoring the anticancer effects of intraluminal plasma-activated solution perfusion. In this paper, the anticancer effects of flowing plasma-activated saline (PAS) on human bladder cancer cell lines (J82) were studied in vitro in a microfluidic system. It is noteworthy that flowing PAS can kill cancer cells more efficiently than PAS immersion. Further experimental results show that the anticancer effects of PAS on J82 cells in the microcavity are affected by plasma irradiation time, perfusion time and liquid flow rate. Our research suggests that PAS perfusion therapy can become a controllable alternative therapy pathway in the future clinical treatment of intracavitary tumors.