Evaluation of Monoraphidium contortum for the tertiary treatment of dairy industry wastewater and biomass production with nitrogen supplementation

The present study sought to evaluate the secondary wastewater from dairy industry as a culture medium for Monoraphidium contortum , in bench-scale tubular photobioreactor, aiming at tertiary wastewater treatment and microalgae biomass production. Since the used secondary wastewater contained residual phosphorus ( P ) but negligible residual nitrogen ( N ), we also evaluated the nitrogen supplementation, following Redfield ratio ( N : P = 16:1) and the same N : P proportion of Bold Medium ( N : P = 1.71:1). These cultures were compared to secondary wastewater without N and Bold Medium (control). Secondary wastewater without N addition provided lower values of maximum biomass concentration, indicating the importance of this supplementation. The nitrogen supplementation following Bold Medium represented the best protocol, since biomass productivity was higher than that in control culture, but with lower nitrogen addition (in comparison with the supplementation following Redfield proportion). The biomass of M. contortum showed to be an excellent candidate for oil production, which could be employed as feedstock for biodiesel, for example.