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抗猪PABPC1蛋白单克隆抗体的制备与鉴定

JIAO Yajuan,WANG Hua,KONG Ning,SUN Dage,DONG Sujie,CHEN Xiaoyong, ZHAI Huanjie, TONG Wu,ZHENG Hao, YU Hai,YU Lingxue,TONG Guangzhi,SHAN Tongling

wf（2022）

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Abstract
细胞质聚腺苷酸结合蛋白1(PABPC1)是一种mRNA poly A结合蛋白,在维持mRNA稳定和蛋白翻译方面起着重要作用.本研究中将猪源PABPC1基因克隆并插入原核表达载体pCold-Ⅰ中,构建重组表达质粒pCold-pPABPC1.将其转化至大肠杆菌BL21(DE3)感受态细胞并经过IPTG诱导表达重组猪PABPC1蛋白.然后,用纯化的rpPABPC1蛋白免疫BALB/c小鼠以制备PABPC1的单克隆抗体.Western blot结果表明,PABPC1单克隆抗体与PK1细胞和HEK293T细胞中的PABPC1蛋白发生了特异性反应.猪PABPC1特异性单克隆抗体为进一步研究PABPC1的功能提供了有价值的工具.
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Key words
PABPC1
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