Transcriptomic Analysis Revealed Antimicrobial Mechanisms of Lactobacillus rhamnosus SCB0119 against Escherichia coli and Staphylococcus aureus .

Lactic acid bacteria were reported as a promising alternative to antibiotics against pathogens. Among them, could be used as probiotics and inhibit several pathogens, but its antibacterial mechanisms are still less known. Here, SCB0119 isolated from fermented pickles could inhibit bacterial growth or even cause cell death in ATCC25922 and ATCC6538, which was mainly attributed to the cell-free culture supernatant (CFS). Moreover, CFS induced the accumulation of reactive oxygen species and destroyed the structure of the cell wall and membrane, including the deformation in cell shape and cell wall, the impairment of the integrity of the cell wall and inner membrane, and the increases in outer membrane permeability, the membrane potential, and pH gradient in and . Furthermore, the transcriptomic analysis demonstrated that CFS altered the transcripts of several genes involved in fatty acid degradation, ion transport, and the biosynthesis of amino acids in , and fatty acid degradation, protein synthesis, DNA replication, and ATP hydrolysis in , which are important for bacterial survival and growth. In conclusion, SCB0119 and its CFS could be used as a biocontrol agent against and .