Brevibacillus daliensis sp. nov., Isolated From Soil in Machangqing Nature Reserve

A new aerobic bacterial strain, designated strain YIM B02290 T , was isolated from the soil of Machangqing, Dali city, Yunnan Province, China. Cells were Gram-stain-positive, sporogenous, rod-shaped, and motile with peritrichous flagella. Strain YIM B02290 T showed the highest 16S rRNA gene sequence similarity with Brevibacillus laterosporus (97.6%) and Brevibacillus halotolerans (97.6%). The ANI and dDDH values between strain YIM B02290 T and the two reference strains Brevibacillus laterosporus LAM00312 T and Brevibacillus halotolerans DSM 25 T are 72.6% and 72.2%, 20.2% and 19.5% based on the draft genome sequence, respectively. The major cellular fatty acids contain anteiso-C 15: 0 and iso-C 15: 0 . The diagnostic diamino acid of the cell-wall peptidoglycan was meso -diaminopimelic acid. The predominant menaquinone was identified as menaquinone-7. The main polar lipids of strain YIM B02290 T were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphatidyl monomethylethanolamine. The genomic DNA G + C content was 40.6 mol%. All results showed that strain YIM B02290 T represents a novel species of the genus Brevibacillus , for which the name Brevibacillus daliensis sp. nov. is proposed. The type strain is YIM B02290 T (= CCTCC AB 2021094 T = CGMCC 1.18802 T = KCTC 43376 T ).