Easy Hi-C: A Low-Input Method for Capturing Genome Organization.
Methods in molecular biology (Clifton, N.J.)(2023)
摘要
Chromosome conformation capture technology and its derivatives have been widely used to study genome organization. Among them, Hi-C (chromosome conformation capture coupling with high-throughput sequencing) is popular in dissecting chromatin architecture on the genome-wide level. However, the intrinsic limitations prevent its application when it comes to rare samples. Here, we present easy Hi-C, a biotin-free technology that dramatically reduces DNA loss and is suitable for low-input samples.
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关键词
Chromatin architecture,Chromosome conformation capture,Deep sequencing,Easy Hi-C,Hi-C,Proximity ligation,Re-linearization,Restriction enzyme digestion,Self-circularization
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