A simplified, amplicon-based method for whole genome sequencing of human respiratory syncytial viruses.

Xiaomin Dong,Yi-Mo Deng,Ammar Aziz,Paul Whitney,Julia Clark,Patrick Harris, Catherine Bautista,Anna Maria Costa,Gregory Waller,Andrew J Daley,Megan Wieringa,Tony Korman,Ian G Barr

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology（2023）

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摘要

This assay is simple to set up, robust, easily scalable in sample preparation and relatively inexpensive, and as such, provides a valuable addition to existing NGS RSV WGS methods.

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关键词

One-step multiplex RT-PCR,Respiratory syncytial virus,Whole genome sequencing

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