Green sensitive spectrofluorimetric determination of pemetrexed as antineoplastic drug; Application in pharmaceutical dosage forms and spiked human plasma.

A recent antineoplastic medication is pemetrexed, this medicine is now being developed and produced on a large scale, thus approaches for quality control are urgently needed. Spectrofluorimetric guidelines for the simple estimation of pemetrexed were validated. Pemetrexed's assay was depends on observations of its native fluorescence at 275/450 nm wavelengths and pH 4. A calibration curve was attained between 1.0 and 80.0 ng mL . To attain maximal sensitivity, at limits of quantitation and detection of 0.903 ng mL and 0.321 ng mL , respectively, various factors impacting the suggested approach were investigated. The proposed approach is thought to be the first spectrofluorimetric one created for pemetrexed estimation. The proposed approach was also used to identify the examined drug in both its formulation and in human plasma that had been spiked. The accuracy and reproducibility of this procedure were confirmed by statistical analysis. To stress the green concept, the ecological influence of the proposed approach was further judged using two newly published evaluation metrics, the AGREE-analytical greenness index and Green Analytical Procedure Index (GAPI).