Chip-based digital Polymerase Chain Reaction as quantitative technique for the detection of PIK3CA mutations in breast cancer patients.

Stefano Giannoni-Luza,Oscar Acosta,Alexis Germán Murillo Carrasco,Pierina Danos,José Manuel Cotrina Concha,Henry Guerra Miller,Joseph A Pinto,Alfredo Aguilar,Jhajaira M Araujo,Ricardo Fujita,Jose Buleje

Heliyon（2022）

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摘要

Digital PCR showed a high sensitivity to detect mutations in tumor samples and it might be capable to detect low-rate mutations and tumor subpopulations not detected by Sanger sequencing.

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关键词

Breast neoplasms,Digital PCR,Genetic techniques,Mutation,PIK3CA,Polymerase chain reaction

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