Canine induced pluripotent stem cells efficiently differentiate into definitive endoderm in 3D cell culture conditions using high-dose activin A

Masaya Tsukamoto,Kazuto Kimura,Takumi Yoshida,Kikuya Sugiura,Shingo Hatoya

Regenerative Therapy（2022）

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摘要

A ciPSC line was successfully differentiated to DE efficiently using a high dose of activin A with knockout serum replacement under 3D cell culture conditions. We believe that this study will be fundamental to achieving the generation of canine endodermal tissues from ciPSCs.

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关键词

3D cell culture,Canine,Definitive endoderm,Endodermal differentiation,Induced pluripotent stem cell

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