Influence of the liver microenvironment on tumor cell growth and immune evasion in liver metastasis of pancreatic cancer

One of the most prevalent cancers in the world and Iran is gastric cancer with a high degree of lethality. There are many environmental factors and genes responsible for gastric cancer incidence. Among genetics factors, NDRG1 has been reported to be associated with the gastric cancer progression, while its single nucleotide polymorphisms (SNPs) have not been widely studied. Among the SNPs, rs1049694 might be of interest as it is located in the 3′UTR of NDRG1 transcripts. However, the relevance of rs1049694 with the incidence of gastric cancer has not been studied yet.In this study, 144 patients with gastric cancer and 66 healthy individuals were selected. After taking blood samples and extraction of genomic DNA, genotyping was performed on 1049694 using allele specific primer (ASP)-PCR method. The data were analyzed with the SNPStats and SPSS.The results showed that 3 different genotypes were found in gastric cancer rs1049694 NDRG1 gene polymorphism. The rs1049694 allele G was significantly associated with the higher risk of gastric cancer and metastasis.The presence of allele G at the rs1049694 position located in NDRG1 gene is significantly associated with the higher risk of gastric cancer. This allele was significantly observed higher in the metastatic patients. This SNP might be studied further to be considered as a biomarker for diagnosis/prognosis of gastric cancer.