Cas9-mediated tagging of endogenous loci using HITAG

To facilitate the interrogation of proteins at scale, we have developed High-throughput Insertion of Tags Across the Genome (HITAG). HITAG enables users to produce libraries of cells, each with a different protein of interest C-terminally tagged, to rapidly characterize protein function. To demonstrate the utility of HITAG, we fused mCherry to a set of 167 stress granule-associated proteins and characterized the factors which drive proteins to strongly accumulate within stress granules. ### Competing Interest Statement A.C. has several issued and provisional patents managed by Harvard University and Columbia University on CRISPR based tools and methods. In addition, AC, JK, and BKS have filed patents related to HITAG with Columbia University.