CRitical Assessment of genomic COntamination detection at several Taxonomic ranks (CRACOT)

Genome contamination is a well known issue in genomics. Although it has already received a lot of attention, with an increasing number of detection tools made available over the years, no comparison between these tools exists in the literature. Here, we report the benchmarking of six of the most popular tools using a simulated framework. Our simulations were conducted on six different taxonomic ranks, from phylum to species. The analysis of the estimated contamination levels indicates that the precision of the tools is not good, often due to large overdetection but also underdetection, especially at the genus and species ranks. Furthermore, our results show that only redundant contamination is accurately estimated. ### Competing Interest Statement The authors have declared no competing interest.