Isolation of Bacterivorous Protozoan, Acanthamoeba Spp., as New-Age Agro Bio-Input

Protozoan is widely distributed in the environment. Acanthamoeba genus are free-living protozoan of nonpathogenic and pathogenic nature. Its intrinsic ability to feed on soil bacteria contributes greatly to the soil nutrient turnover and thereby plant growth. This protocol provides a firsthand guide to isolate Acanthamoeba from soil samples and their preliminary identification through staining procedure. The isolates could be further tested for plant beneficial roles in laboratory settings.