Epidemiological Survey of the Main Tick-Borne Pathogens Infecting Dogs from the Republic of Moldova.

Despite the significant burden of tick-borne diseases (TBDs), epidemiologic studies are missing, and TBD awareness is low in the Republic of Moldova. Our study is the first to assess the prevalence of the main tick-borne pathogens (TBPs) infecting dogs in this country and associated risk factors. In this cross-sectional, multi-centre study (June 2018-July 2019), blood samples were collected from dogs presenting in veterinary clinics (Chişinău: N = 30) and hosted in public dog shelters (Cahul: N = 42; Chişinău: N = 48). TBPs were assessed by molecular techniques and risk factors by the logistic regression model. was the most prevalent TBP (15.8% [19/120]), followed by (11.7% [14/120]), (5.8% [7/120]), and spp. (0.8% [1/120]). Blood samples tested negative for spp., spp., , , and . Dogs originating from the veterinary clinics had a higher prevalence of infection than those from the shelters (16.6% versus 2.2%, respectively, = 0.0292; OR: 27.0 [95%CI: 1.4-521.9]). Dogs from Chișinău had a higher prevalence of infection versus those from Cahul (19.2% versus 9.5%, respectively, = 0.0295; OR: 3.9 [95%CI: 1.1-13.4]). We recommend routine use of acaricides and deworming of dogs to prevent or/and limit TBD spread. Further TBD surveillance studies are needed.