Subgenomic RNA abundance relative to total viral RNA among SARS-CoV-2 variants

Maxwell Su, Sara Ping,Phuong-Vi Nguyen,Alejandra Rojas,Laila Hussaini,Ludy Registre Carmola,Azmain Taz,Julie Sullivan,Greg S Martin,Anne Piantadosi,Magaly Martinez,Wilbur A Lam,Evan J Anderson,Jesse J Waggoner

Open Forum Infectious Diseases（2022）

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摘要

Abstract SARS-CoV-2 subgenomic RNA (sgRNA) may indicate actively replicating virus, but sgRNA abundance has not been systematically compared between SARS-CoV-2 variants. sgRNA was quantified in 169 clinical samples by rRT-PCR, demonstrating similar relative abundance among known variants. Thus, sgRNA detection can identify individuals with active viral replication regardless of variant.

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