Comparative Analysis of Bioinks in 3D Bioprinted Organoids of Colorectal Cancer

Colorectal cancer (CRC) is the third most common and fourth deadliest cancer in Western countries.Despite treatment regimens, the number of deaths increases yearly, particularly among younger patients.Current therapies are insufficient due to the heterogenetic nature of CRC and demand new strategies for personalized treatment.The aim of our pilot study was to develop 3D organoids utilizing bioinks suitable for extrusion 3D bioprinting of CRC cell lines.We focused on the characterization of 3D printed organoids based on bioink properties.We assessed cell viability and growth patterns by fluorescence microscopy.3D CRC cells printed in different bioinks revealed 90 % viability up to seven days after printing showing various cell growth patterns.In conclusion, our work demonstrates the immense opportunities of 3D bioprinting to generate tumor organoids.We proved that alginate and gelatin-based bioinks mixed with live cells are suitable for extrusion 3D bioprinting.