Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics
npj Biofilms and Microbiomes(2022)
Abstract
The intestinal epithelial barrier facilitates homeostatic host-microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex-vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.
### Competing Interest Statement
The authors have declared no competing interest.
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Key words
Biological techniques,Symbiosis,Life Sciences,general,Microbiology,Medical Microbiology,Microbial Ecology,Microbial Genetics and Genomics
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