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RECOGNITION OF SODIUM-DEPENDENT PHOSPHATE TRANSPORTER NAPI2B BY MONOCLONAL ANTIBODIES IN BACTERIAL AND EUKARYOTIC CELLS

L.F. Bulatova,R.G. Kiyamova

BIOTECHNOLOGY: STATE OF THE ART AND PERSPECTIVES（2022）

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Abstract
The membrane sodium-dependent phosphate transporter NaPi2b is attractive target for monoclonal antibody therapy. Recognition of the large extracellular domain (ECD) of NaPi2b by monoclonal antibodies L2(20/3) in Western blot analysis depends on the addition of DTT to lysates of eukaryotic cells, but not bacterial cells.
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antibodies,bacterial,sodium-dependent
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