Function of Connexin 43 and RhoA/LIMK2/Cofilin Signaling Pathway in Transient Changes of Contraction and Dilation of Human Umbilical Arterial Smooth Muscle Cells
International journal of biochemistry & cell biology(2022)
摘要
Background: Post-induction hypotension, a common complication after propofol-based induction regimen, is a life-threatening challenge for anesthesiologists especially when unexpected pre-induction hypertension charac-terized by angiotensin release and increased vascular tone was presented by the same patient. Gap junctions (GJs) composed of connexin 43 (Cx43) have been considered a key factor in regulating vascular contraction and dilation. We aimed to explore the role of Cx43-GJs during peri-induction blood pressure fluctuation and elucidate the underlying mechanisms. Methods: Human umbilical arterial smooth muscle cells (HUASMCs) were pretreated by short-term Angiotensin II (Ang II) with or without subsequent propofol treatment to simulate transient contraction and dilation of vascular smooth muscle cells during anesthesia induction. F-actin polymerization, a classic indicator of HUASMCs constriction, was determined by F-actin staining assay. Both the function and expression of Cx43-GJs during transient contraction and dilation of HUASMCs, and their potential regulation of downstream Ca2+/RhoA/ LIMK2/Cofilin signaling pathway were explored via different targeting inhibitors and siRNAs. Results: Ang II pretreatment significantly induced F-actin polymerization that indicate cell contraction, accom-panied by enhanced GJs function on HUASMCs. With the inhibition of Cx43 GJs by the specific inhibitor, Gap26, and Cx43-siRNA, Ang II-induced F-actin polymerization was reversed accompanied with the decrease of intra-cellular Ca2+ mobility and the RhoA/LIMK2/Cofilin signaling pathway activity. We also noticed that propofol application could inhibit GJs function, the same as Gap26. Simultaneously, intracellular Ca2+ mobility and RhoA/LIMK2/Cofilin signaling pathway activity on HUASMCs were both downregulated, finally resulting in downstream reduction of F-actin polymerization. Conclusion: The function of Cx43-GJs lies in the center of Ang II-induced contraction of HUASMCs, which potentially regulates intracellular Ca2+ mobility as well as RhoA/LIMK2/Cofilin signaling pathway activity. Propofol can reverse this effect induced by Ang II through suppressing the function of Cx43-GJs.
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关键词
Angiotensin II,Propofol,Gap junction,Connexin 43
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