A novel method to assess copy number variation in melanoma: Droplet digital PCR for precise quantitation of the RREB1 gene in formalin-fixed, paraffin-embedded melanocytic neoplasms, a proof-of-concept study.

Keegan O'Hern,Rachael Barney,Meagan Chambers,Catherine Baker,Mirjana Stevanovic,Gregory J Tsongalis,Edward Hughes,Aravindhan Sriharan

Journal of cutaneous pathology（2023）

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摘要

Here we show that ddPCR can provide inexpensive, rapid, and robust data on the commonest copy number alteration in melanoma. Future development and validation could provide a useful ancillary tool in the diagnosis of challenging melanocytic lesions.

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关键词

RREB1,droplet digital PCR,melanocytic nevus,melanoma

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