Biodegradability of polyethylene by efficient bacteria from the guts of plastic-eating waxworms and investigation of its degradation mechanism

Polyethylene (PE) has been regarded as non-biodegradable for decades, and the evidence for its degradation by bacteria remains unclear in the literature. Waxworms have recently gained attention for their ability to degrade natural long-chain polymers and synthetic plastic. This study aims to explore the potential of low-density polyethylene (LDPE)-degrading bacteria from the gut symbionts of lesser waxworm (Achroia grisella) larvae for the effective biodegradation of LEDP. Two bacterial isolates (LDPE-DB1 and LDPE-DB2) exhibited the greatest reduction in tensile strength among all isolates (P < 0.0001), reaching 51.3% and 58.3%, respectively. The bacterial strains LDPE-DB1 and LDPE-DB2 stand for molecularly identified species, Citrobacter freundii and Bacillus sp., respectively. After 5 days of incubation, the cell density of LDPE-DB1 and LDPE-DB2 reached 2.20 x 10(8) and 1.8 x 10(8) CFU/mL, respectively. However, after 30 days of incubation, the cell density reached 7.3 x 10(8) and 5.9 x 10(8), respectively. The formed cavities indicate the high activity of the isolated bacteria from Achroia grisella larvae where the cavities reach a depth of up to 1.2 mu m. The findings of this study demonstrated the presence of LDPE-degrading bacteria in Achroia grisella and provide promising evidence for the biodegradation of plastic waste management in the environment.