Automatic cell type annotation using supervised classification: A systematic literature review

Single-cell sequencing gives us the opportunity to analyze cells on an individual level rather than at a population level. There are different types of sequencing based on the stage and portion of the cell from where the data are collected. Among those Single Cell RNA seq is most widely used and most application of cell type annotation has been on Single-cell RNA seq data. Tools have been developed for automatic cell type annotation as manual annotation of cell type is time-consuming and partially subjective. There are mainly three strategies to associate cell type with gene expression profiles of single cell by using marker genes databases, correlating expression data, transferring levels by supervised classification. In this SLR, we present a comprehensive evaluation of the available tools and the underlying approaches to perform automated cell type annotations on scRNA-seq data.