Assigning Functions of Unknown Enzymes by High-Throughput Enzyme Characterization.
Methods in molecular biology (Clifton, N.J.)(2023)
Abstract
The discovery of new enzymes is strongly enabled by the implementation of high-throughput screening methods to detect enzymatic activity in single organisms or clone expression libraries, or to benchmark their performances against known prototypes. In this chapter, a number of methods, applicable at high-throughput scale, are described that allow the screening and characterization of enzymes relevant to biotechnology, particularly, ester-hydrolases (esterases, lipases, phospholipases, and polyester hydrolases).
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Key words
Enzyme characterization,Ester-hydrolases,Esterase,High-throughput assays,Lipase,Metagenomics
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