The influence of proteoforms: assessing the accuracy of total vitamin D-binding protein quantification by proteolysis and LC-MS/MS.

Lisa E Kilpatrick,Roger Bouillon,W Clay Davis,Clark M Henderson,Andrew N Hoofnagle,Steven Pauwels,Dirk Vanderschueren,Etienne Waelkens,Hans Wildiers,James H Yen,Karen W Phinney

Clinical chemistry and laboratory medicine（2023）

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摘要

VDBP proteoforms were not found to cause bias during quantification by LC-MS/MS, thus demonstrating that a family of proteins can be accurately quantified using shared peptides. A reference value was assigned for total VDBP in SRM 1950, which may be used to standardize methods and improve the accuracy of VDBP quantification in research and clinical samples.

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关键词

LC-MS/MS,proteoform,quantification,reference value,vitamin D-binding protein

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