Standardizing Clinical Workflow for Assessing Minimal Residual Disease by Flow Cytometry in Multiple Myeloma

David M Foureau,Barry A Paul,Fei Guo,Edward H Lipford,Kateryna Fesenkova,Elise Tjaden,Kendra Drummond,Manisha Bhutani,Shebli Atrash,Ami Ndiaye,Cindy Varga,Peter M Voorhees,Saad Z Usmani

Clinical Lymphoma Myeloma and Leukemia（2023）

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摘要

•Technical 1st pull bone marrow aspirate yields adequate specimens for MRD testing.•MRD testing by flow cytometry can routinely achieve 1-2 × 10−6 analytic sensitivity.•Low assay input and hemodilution quantifiably impact MRD assay sensitivity.•Specimen adequacy is an important element of MRD status reporting.

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关键词

Multiple myeloma MRD,Bone marrow collection,Multicolor cytometry assay performance,Reporting

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