Effects of rhamnolipid pretreatment on DOM dissolution characteristics and anaerobic fermentation acid production of waste activated sludge

In this paper, the change characteristics of DOM (dissolved organic matter) and acid production characteristics of anaerobic fermentation in waste activated sludge(WAS) pretreated with rhamnolipid (RL) were studied. The results showed that RL at the dose of 80 mg/gTS could significantly promote the disintegrating of EPS (extracellular polymers) and cell wall in WAS, and a large number of proteins and carbohydrates were dissolved. Three dimensional fluorescence parallel factor analysis showed that the addition of RL enhanced the dissolution and biodegradability of humus-like substances. LC-OCD (Liquid chromatography - organic carbon detection) analysis showed that RL promotes the dissolution of biodegradable components such as Biopolymer, Building Blocks and LMW Neutrals, and ensures the increase of VFA (volatile fatty acids) production in the process of anaerobic fermentation. Under the RL dose of 80 mg/gTS, the maximum VFA production of WAS was obtained at 108 h of anaerobic fermentation, which was 2699.39 mg/L. Acetic acid and propionic acid were the main components in the WAS fermentation broth pretreated by RL. The concentration of butyric acid increased with the increase of RL dose. The RL dose can significantly affect the composition of VFA in WAS fermentation broth.