IN-VITRO EVALUATION OF ANTHELMINTIC ACTIVITY AND MEMBRANE-STABILIZING POTENTIAL OF Randia Dumetorum LEAF EXTRACT

This study reports the anthelmintic potential and membrane stabilization activity of distinct fractions (methanol, chloroform, and n-hexane extracts) using Soxhlet extractor of Randia dumetorum (R. dumetorum) leaf extracts. Paralyzed time and death time as significant indicators were used for monitoring anthelmintic activity of the examined extracts and compared with standard albendazole drug, which is applied against Bangladeshi adult earthworm (Pheretima posthutna). Human red blood cells were used to prove the efficacy of stability activity of the examined extracts based on hypotonic solution condition, whereas acetyl salicylic acid (ASA) was used as standard anti-inflammatory drug to compare the potentiality of extracts. Among three concentrations (10, 20, and 40 mg/mL) of the examined extracts, the n-hexane extract of leaf showed the best anthelmintic activity (time of paralysis 9.44 min and time of death 22.30 min) under investigation at 40 mg/mL. Significant variation (p < 0.05) was observed when compared with the standard. The chloroform extract recorded the highest membrane-stabilizing activity, which showed the highest inhibition (78.37 +/- 0.08%), compared with methanol (50.96 +/- 0.05%) and n-hexane (42.86 +/- 0.06%). This result was attributed to the existence of the significant content of flavonoids in chloroform extract showing considerable and effective anti-inflammatory activities in vitro, which was compared with ASA (54.59 +/- 0.03%).