Pollen germination and hand pollination in pitaya (Selenicereus spp.)

Hand pollination is a necessary assisting method for pitaya (Selenicereus spp.) production to achieve a high yield. With the cultivated area increasing at an exponential rate in recent years, a comprehensive study of the pollination process was conducted. We developed an ideal medium for pitaya pollen in vitro germination in this study, then tested the activity of pollen collected from or stored for various time periods. We discovered that those collected between 2 h before blooming and 6 h after blooming had the higher germination rates (27.2???65.1%), the highest activity of pollen occurred between 2 h and 4 h after blooming, and that storing them at 4??C for 24 h reduces their germination rate from 65.2 percent to 35.5 percent and their production to about 82%. As a result, pollinating plants with pollen that has been held for more than 24 h is not recommended unless a breakthrough in pollen storage is achieved. We also discovered that stigma receptivity and pollen activity are synchronized, which together determines the rate of fruit setting and the size of the fruit. Pollination within 6 h after flowering offers the optimum fruit setting percentage and size, while pollination at 6:00 pm, 2 h before blooming, is also a good alternative; however, pollination at 6:00 am the next morning is expected to result in a 23% drop in the fruit size. These findings will be beneficial for reproductive biology research, as well as laying the groundwork for hand pollination to boost pitaya output and breeding efficiency.