The effect of two calcium phosphate-containing agents on the enamel resistance of permanent molars to demineralization: An experimental study

Background: The main purpose of this experimental study was to determine the in vitro effects of two calcium phosphate-containing agents (Remin Pro (R) and GC Tooth mousse (TM)) on the enamel resistance of permanent molars to demineralization. Methods: Fifty extracted human third molars were randomly divided into four groups; that is the control group and three case groups treated with Remin Pro (R), GC Tooth mousse (TM), and sodium fluoride gel. The three case groups were treated with 0.25 ml of the paste associated with each experimental group for 5 min, kept in fluoride-free artificial saliva, and incubated at 37 degrees C for 28 days. After the treatment regimen, 10 samples of each case group were subjected to demineralization using an acetic acid-containing solution, and remineralization using a remineralizing solution. The morphology of enamel was observed via scanning electron microscopy and their enamel calcium/phosphorus (Ca/P) ratios were measured before/after the demineralization cycle with energy-dispersive X-ray analysis. Data were statistically analyzed using one-way analysis of variance and post hoc Tukey tests. Results: The enamel Ca/P ratios in the case study groups were significantly higher than that of the control group before/after the demineralization regimen (p < .0001). However, the ratios were not significantly different between the case study groups after the treatment regimen and demineralization cycle (p > .05). Conclusion: The outcomes of the current study indicated that all three agents seemed to increase the enamel resistance of permanent molar teeth to demineralization.