Effects of molecular weight on intestinal anti-inflammatory activities of beta-D-glucan from Ganoderma lucidum

To investigate the influence of molecular weight (M-w) on the anti-inflammatory activity of beta-D-glucan from Ganoderma lucidum, ultrasonic irradiation was applied to treat the beta-D-glucan (GLP, 2.42 x 10(6) g/mol) solution to obtain two degraded fractions with molecular weight of 6.53 x 10(5) g/mol (GLPC) and 3.49 x 10(4) g/mol (GLPN). Structural analysis proved that the degraded fractions possessed similar repeated units with the original beta-D-glucan. The in vitro anti-inflammatory activity studies showed that all fractions could significantly inhibit LPS-induced expression of cytokines including TNF-alpha, IL-8, MIF and MCP-1 in Caco-2 cells at certain concentrations. Moreover, GLPC and GLPN exhibited better anti-inflammatory activity than GLPC. The intestinal anti-inflammatory activity evaluated by dextran sulfate sodium (DSS)-induced colitis mice model showed that intragastric administration of GLPN (lower M-w fraction) could significantly recover inflamed tissues of mice. Compared with GLP and GLPC, GLPN exhibited stronger ability to inhibit the secretion of pro-inflammatory cytokines (TNF-alpha, IL-1 beta, and IL-6). The results revealed that M-w of beta-D-glucan influenced its anti-inflammatory activity and decreasing of M-w would improve the activity, which provided evidence for the potential use of beta-B-glucan from G. lucidum as anti-colitis ingredients.