A circadian-like gene network regulates heterochronic miRNA transcription in C. elegans
bioRxiv (Cold Spring Harbor Laboratory)(2023)
Abstract
Developmental robustness relies on precise control of the timing and order of cellular events. In C. elegans , the invariant sequence of post-embryonic cell fate specification is controlled by oscillatory patterns of heterochronic microRNA transcription that are phase-locked with the larval molting cycle[1][1]-[4][2]. How these transcriptional patterns are generated and how microRNA dosage is controlled is unknown. Here we show that transcriptional pulses of the lin-4 heterochronic microRNA are produced by two nuclear hormone receptors, NHR-85 and NHR-23, whose mammalian orthologs, Rev-Erb and ROR, function in the circadian clock. While Rev-Erb and ROR play antagonistic roles in regulating once-daily transcription[5][3]-[7][4], we find that NHR-85 and NHR-23 bind cooperatively as heterodimers to lin-4 regulatory elements to induce a single brief pulse of expression during each larval stage. We demonstrate that the timing and duration of lin-4 transcriptional pulses are programmed by the phased overlap of NHR-85 and NHR-23 protein expression and that these regulatory interactions are post-transcriptionally controlled by LIN-42, the circadian Period ortholog in C. elegans . These findings suggest that an evolutionary rewiring of the circadian clock machinery is co-opted in nematodes to generate periodic transcriptional patterns that define cell fate progression.
### Competing Interest Statement
The authors have declared no competing interest.
[1]: #ref-1
[2]: #ref-4
[3]: #ref-5
[4]: #ref-7
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Key words
heterochronic mirna transcription,gene,circadian-like
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