Benign Production of AgNPs/Bacterial Nanocellulose for Wound Healing Dress: Antioxidant, Cytotoxicity and In Vitro Studies

New facile way for production of antimicrobial wound healing dress was achieved using freshly fabricated bacterial cellulose (BNC) decorated with in situ formed silver nanoparticles (AgNPs). Herein, BNC was fabricated by using a domestic isolated strain Komagataeibacter hansenii SA1.1 . BNC/AgNPs nanocomposites were in situ synthesized by introducing AgNO 3 to alkaline BNC at 60 °C for 30 min to attain full chemical reduction of Ag ions which manifested visually by color change. Results signified the successful fabrication of nanofibrillated BC with network structure. Also, AgNPs were successfully assembled on the surface of BNC in spherical shape with distribution uniformity consists of narrow size particles lied in range of 10–40 nm. The hybrid BNC/AgNPs manifested a strong antibacterial activity against Gram negative and Gram positive as well as unicellular fungus. The results from in vitro study demonstrated, undoubtedly, that BNC/AgNPs can be able to promote the wound healing affinity of chronic ulcers through reducing microbial infection besides accelerating the healing by their inflammatory. While, the antioxidant affirmed the potential protection of BNC/AgNPs from oxidative stressors, it was displayed a vital role in the improvement cell propagation and cell proliferation.