Deep ultraviolet based serial block-face imaging for 3-dimensional morphological assessment of the rodent brains

We present a deep ultraviolet (DUV) light microscope capable of serial block-face imaging of the whole rodent brain. The capability of the 3D-DUV microscope for quantitative volumetric analysis of the small substructures of habenula is shown. It is expected that large scale wide-field high resolution analysis of the smaller regions would allow for elucidating the functional changes linked with the morphological changes in brain under pathological conditions.