Investigation of structure–stability correlations of reconstructed oil bodies

To overcome the challenges of complex synthesis methods and the thermodynamic instability associated with conventional emulsions, the artificial oil bodies (AOBs) containing oil body proteins (OBPs) and phospholipids (PLs) were fabricated using ultrasonication and the correlations between their reconstituted structures and characteristics were investigated. As the PL concentration was increased, the concentrations of α-helix and β-turns in interfacial proteins decreased, whereas those of β-sheets and random coils increased. Moreover, the number of the intermolecular hydrogen bond first increased and then decreased. At an OBP/PL ratio of 0.5, the AOBs exhibited the lowest mean particle size (unimodal distribution), highest creaming index, and lowest KE. The oxidation stabilities of the AOBs with OBP/PL ratios of 0.05 and 0.015 were higher than those of other AOBs. Furthermore, statistical analysis indicated that the secondary structure and tyrosine residues of the interface protein were significantly correlated with the stability of the AOBs (p < 0.05), but the hydrogen bond of the interface protein has a low correlation with the stability of the oil body.