Genome sequencing of an XDR Klebsiella pneumoniae ST101 strain isolated from a Brazilian Amazon river

OBJECTIVE:Herein, this study aimed to perform the genomic characterization of a bla KPC-2METHODS:Antimicrobial susceptibility testing was performed following BrCAST/EUCAST recommendations. Genomic DNA was extracted and sequenced using the Illumina® NextSeq platform and the assembly of the generated reads was performed using the SPAdes software. Research on the sequence type, resistance and virulence encoding genes, and plasmid replicon typing was carried out. RESULTS:The KP1.1JP strain was resistant to all β-lactams, aminoglycosides, and fluoroquinolones tested. The genome size was 5 626 346 bp, distributed in 203 contigs and a guanine and cytosine content of 57.02%. The values of N 5075CTX-M-15TEM-1BOXA-1SVH-182,KPC-2CONCLUSIONS:We report the occurrence of a K. pneumoniae ST101 strain carrying bla KPC-2