Three new eremophilane sesquiterpenes and one new related derivative from Nemania sp. HDF-Br-5, an endophytic fungus of the endangered conifer Pseudotsuga gaussenii Flous

Three new (psganpenes A-C), one known eremophilane sesquiterpene and one newrelated derivative (psganpene D) were isolated from the culture of endophytic fungus Nemania sp. HDF-Br-5, which derived from Pseudotsuga gaussenii Flous, anendangered conifer endemic to China. Their structures were deduced by extensive spectroscopic methods. All isolates were screened for cytotoxicity and antibacterial activity. Psganpene D showed promising cytotoxicity against KB, HCT116 and Hela cell strains, with IC50 values in the range of 15.1-32.3 mu M.