Antinociceptive activity of Pereskia sacharosa Griseb. (Cactaceae) and localization of bioactive compounds in mice brain

•Micropropagation of P. sacharosa is an alternative for the production of plants.•Kaempferol is a constituent of P. sacharosa.•Isolated fractions showed a stronger analgesic effect than the crude extracts of P. sacharosa.•The antagonist showed an antinociceptive effect in coadministration with P. sacharosa isolated compounds.•Bioactive metabolites of P. sacharosa were successfully detected by epifluorescence in mice brain.