Multiplexed single-cell RNA-sequencing of mouse thymic and splenic samples
STAR Protocols(2022)
摘要
Multiplexed single-cell RNA-sequencing (scRNA-seq) enables investigating several biological samples in one scRNA-seq experiment. Here, we use antibodies tagged with a hashtag oligonucleotide (Ab-HTO) to label each sample, and 10× Genomics technology to analyze single-cell gene expression. Advantages of sample multiplexing are to reduce the cost of scRNA-seq assay and to avoid batch effect. It may also facilitate cell-doublet removal and the merging of several scRNA-seq assays. Herein, we apply multiplexed scRNA-seq to investigate mouse thymocytes and splenic T lymphocytes development.
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关键词
Bioinformatics,Sequence analysis,Single Cell,Flow Cytometry/Mass Cytometry,Sequencing,RNAseq,Immunology,Antibody
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