Hydroxypropyl-8-cyclodextrin/Oridonin and Trehalose loaded nanovesicles attenuate foam cells formation and regulate the inflammation

Hydroxypropyl-8-Cyclodextrin (HP -8 -CD) was used to form the HP-8-CD/Oridonin inclusion complex. Then, the novel Multi-loaded nanovesicles systems (NVs) were established to encapsulate both hydrophilic (Trehalose) and hydrophobic drugs (Oridonin inclusion complex) with peptides. NVs had an average size of 224.4 +/- 10.84 nm and a zeta potential of 17.83 +/- 1.05 mV. Furthermore, these NVs significantly decreased lipid over -accumulation, and reverse the formation of foam cells in RAW264.7 cells. Oridonin and Trehalose in NVs pro-vided significant synergistic effects to inhibit lipid over-accumulation (CI < 1). In addition, NVs could modulate the release of IL-18, IL-6 and TNF-a, promote the formation of autophagosomes. Taken together, the Multi-loaded NVs may be used as an effective drug delivery system, providing new insights into decrease the formation of foam cells and regulate the inflammation.