A nested RT-PCR method of HCV RNA core protein gene testing and genotyping for entry-exit people

Objective To establish a method of nested RT-PCR to test HCV core protein gene and genotype.Methods HCV core protein gene sequence was searched from the Hepatitis C Virus(HCV) Database and HCV gene premier was designed for nested RT-PCR reaction system and genotyping.A total of 60 anti-HCV positive serum,50 anti-HCV negative and alanine aminotransferase(ALT) higher than 100U/L serum from entry-exit people were tested.Sequencing the RT-PCR reactive products for HCV genotype.Results From 60 anti-HCV positive serum,42(70%) were HCV core protein gene positive,including 27 lb genotype,12 la genotype,1 for 6a,3a,and 2a genotype.For 50 anti-HCV negative and ALT higher than 100 U/L serum,1 was 3a genotype.Conclusion This study established a quick and accurate method to test HCV core protein gene and genotype.